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draft genome sequence of l. maculans isolate jn3  (Genoscope)

 
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    Genoscope draft genome sequence of l. maculans isolate jn3
    A) The cpcA locus of Leptosphaeria <t>maculans</t> . The conserved leucine zipper region at the C-terminus of cpcA is dark grey. The open boxes indicate the upstream Open Reading Frames uORF1 and uORF2 in the 5' leader region. The black dot preceding them represents the putative cross-pathway control element (CPRE) whose sequence is 5'TGACTCA3'. B) Alignment of the deduced amino acid sequence of uORF2 with counterparts in the leader sequences of Aspergillus fumigatus (Af) cpcA (GenBank XP_751584.1 ) and A. nidulans (An) cpcA (GenBank AF302935 ). Black boxes with white text denote amino acids identical in two of the three fungal species. Grey boxes with white text mark conserved changes. Gaps are introduced to optimize alignment. C) Alignment of the deduced amino acid sequence of the C-terminus conserved leucine zipper region with that of A. fumigatus CpcA and A. nidulans CpcA. The thick black line denotes the bZIP transcription factors basic domain signature (PS00036). Asterisks mark positions where conserved leucine residues characteristic of a leucine zipper (L-x(6)-L-x(6)-L-x(6)-L) should be found.
    Draft Genome Sequence Of L. Maculans Isolate Jn3, supplied by Genoscope, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/draft+genome+sequence+of+l%2E+maculans+isolate+jn3/l++maculans+whole+genome+sequence/pmc03199737-193-14-18
    Average 90 stars, based on 1 article reviews
    draft genome sequence of l. maculans isolate jn3 - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "The cross-pathway control system regulates production of the secondary metabolite toxin, sirodesmin PL, in the ascomycete, Leptosphaeria maculans"

    Article Title: The cross-pathway control system regulates production of the secondary metabolite toxin, sirodesmin PL, in the ascomycete, Leptosphaeria maculans

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-11-169

    A) The cpcA locus of Leptosphaeria maculans . The conserved leucine zipper region at the C-terminus of cpcA is dark grey. The open boxes indicate the upstream Open Reading Frames uORF1 and uORF2 in the 5' leader region. The black dot preceding them represents the putative cross-pathway control element (CPRE) whose sequence is 5'TGACTCA3'. B) Alignment of the deduced amino acid sequence of uORF2 with counterparts in the leader sequences of Aspergillus fumigatus (Af) cpcA (GenBank XP_751584.1 ) and A. nidulans (An) cpcA (GenBank AF302935 ). Black boxes with white text denote amino acids identical in two of the three fungal species. Grey boxes with white text mark conserved changes. Gaps are introduced to optimize alignment. C) Alignment of the deduced amino acid sequence of the C-terminus conserved leucine zipper region with that of A. fumigatus CpcA and A. nidulans CpcA. The thick black line denotes the bZIP transcription factors basic domain signature (PS00036). Asterisks mark positions where conserved leucine residues characteristic of a leucine zipper (L-x(6)-L-x(6)-L-x(6)-L) should be found.
    Figure Legend Snippet: A) The cpcA locus of Leptosphaeria maculans . The conserved leucine zipper region at the C-terminus of cpcA is dark grey. The open boxes indicate the upstream Open Reading Frames uORF1 and uORF2 in the 5' leader region. The black dot preceding them represents the putative cross-pathway control element (CPRE) whose sequence is 5'TGACTCA3'. B) Alignment of the deduced amino acid sequence of uORF2 with counterparts in the leader sequences of Aspergillus fumigatus (Af) cpcA (GenBank XP_751584.1 ) and A. nidulans (An) cpcA (GenBank AF302935 ). Black boxes with white text denote amino acids identical in two of the three fungal species. Grey boxes with white text mark conserved changes. Gaps are introduced to optimize alignment. C) Alignment of the deduced amino acid sequence of the C-terminus conserved leucine zipper region with that of A. fumigatus CpcA and A. nidulans CpcA. The thick black line denotes the bZIP transcription factors basic domain signature (PS00036). Asterisks mark positions where conserved leucine residues characteristic of a leucine zipper (L-x(6)-L-x(6)-L-x(6)-L) should be found.

    Techniques Used: Control, Sequencing

    Quantitative Reverse Transcription PCR analysis of (A) cpcA, trpC and aroC , (B) sirZ and sirP in wild type (wt) and a cpcA -silenced (cpcA-sil) isolate of Leptosphaeria maculans . Six replicates of each isolate were grown in Tinline for eight days and then mycelia were washed and then transferred to fresh Tinline media for 5 h with 5 mM 3AT (+) or without 3AT (-). RNA was isolated from all treatments, cDNA prepared and q RT-PCR carried out. Transcript level is normalised to that of actin. Values are means ± SE of triplicate reactions of three independent biological samples. Asterisks mark values that have a significant increase (p < 0.05) in mean transcription levels compared to controls without 3AT.
    Figure Legend Snippet: Quantitative Reverse Transcription PCR analysis of (A) cpcA, trpC and aroC , (B) sirZ and sirP in wild type (wt) and a cpcA -silenced (cpcA-sil) isolate of Leptosphaeria maculans . Six replicates of each isolate were grown in Tinline for eight days and then mycelia were washed and then transferred to fresh Tinline media for 5 h with 5 mM 3AT (+) or without 3AT (-). RNA was isolated from all treatments, cDNA prepared and q RT-PCR carried out. Transcript level is normalised to that of actin. Values are means ± SE of triplicate reactions of three independent biological samples. Asterisks mark values that have a significant increase (p < 0.05) in mean transcription levels compared to controls without 3AT.

    Techniques Used: Reverse Transcription, Isolation, Reverse Transcription Polymerase Chain Reaction

    Sirodesmin PL levels in culture filtrates of in wild type (wt) and a cpcA -silenced (cpcA-sil) isolate of Leptosphaeria maculans . Cultures were grown for eight days in Tinline media (8d) and the culture filtrate isolated and sirodesmin PL levels were quantified by HPLC. Mycelia were washed then transferred to fresh Tinline medium with water (+H 2 O) or 5 mM 3AT (+3AT), or Tinline medium with no carbon or nitrogen sources (-C/N) for a further eight days. Culture filtrates from the three treatments (+H 2 O, +3AT, -C/N) were extracted and sirodesmin PL levels were quantified by HPLC. Values are means ± SE of three independent biological samples. Asterisks mark values that have a significant increase or decrease (p < 0.05) in sirodesmin PL production compared to water controls (+H 2 O).
    Figure Legend Snippet: Sirodesmin PL levels in culture filtrates of in wild type (wt) and a cpcA -silenced (cpcA-sil) isolate of Leptosphaeria maculans . Cultures were grown for eight days in Tinline media (8d) and the culture filtrate isolated and sirodesmin PL levels were quantified by HPLC. Mycelia were washed then transferred to fresh Tinline medium with water (+H 2 O) or 5 mM 3AT (+3AT), or Tinline medium with no carbon or nitrogen sources (-C/N) for a further eight days. Culture filtrates from the three treatments (+H 2 O, +3AT, -C/N) were extracted and sirodesmin PL levels were quantified by HPLC. Values are means ± SE of three independent biological samples. Asterisks mark values that have a significant increase or decrease (p < 0.05) in sirodesmin PL production compared to water controls (+H 2 O).

    Techniques Used: Isolation

    Related Articles

    Sequencing:

    Article Title: The cross-pathway control system regulates production of the secondary metabolite toxin, sirodesmin PL, in the ascomycete, Leptosphaeria maculans
    Article Snippet: These flanking regions were each cloned into plasmid pCR ® 2.1-TOPO (Invitrogen). .. The sequences of the resulting plasmids were compared to the draft genome sequence of L. maculans isolate JN3 (Genoscope and Unité de Recherche Génomique Info, France) and 10 kb regions flanking these DNA fragments were analysed by FGENESH for presence of ORFs. ..



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    draft genome sequence of l. maculans isolate jn3  (Genoscope)
    90
    Genoscope draft genome sequence of l. maculans isolate jn3
    A) The cpcA locus of Leptosphaeria <t>maculans</t> . The conserved leucine zipper region at the C-terminus of cpcA is dark grey. The open boxes indicate the upstream Open Reading Frames uORF1 and uORF2 in the 5' leader region. The black dot preceding them represents the putative cross-pathway control element (CPRE) whose sequence is 5'TGACTCA3'. B) Alignment of the deduced amino acid sequence of uORF2 with counterparts in the leader sequences of Aspergillus fumigatus (Af) cpcA (GenBank XP_751584.1 ) and A. nidulans (An) cpcA (GenBank AF302935 ). Black boxes with white text denote amino acids identical in two of the three fungal species. Grey boxes with white text mark conserved changes. Gaps are introduced to optimize alignment. C) Alignment of the deduced amino acid sequence of the C-terminus conserved leucine zipper region with that of A. fumigatus CpcA and A. nidulans CpcA. The thick black line denotes the bZIP transcription factors basic domain signature (PS00036). Asterisks mark positions where conserved leucine residues characteristic of a leucine zipper (L-x(6)-L-x(6)-L-x(6)-L) should be found.
    Draft Genome Sequence Of L. Maculans Isolate Jn3, supplied by Genoscope, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/draft+genome+sequence+of+l%2E+maculans+isolate+jn3/l++maculans+whole+genome+sequence/pmc03199737-193-14-18
    Average 90 stars, based on 1 article reviews
    draft genome sequence of l. maculans isolate jn3 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A) The cpcA locus of Leptosphaeria maculans . The conserved leucine zipper region at the C-terminus of cpcA is dark grey. The open boxes indicate the upstream Open Reading Frames uORF1 and uORF2 in the 5' leader region. The black dot preceding them represents the putative cross-pathway control element (CPRE) whose sequence is 5'TGACTCA3'. B) Alignment of the deduced amino acid sequence of uORF2 with counterparts in the leader sequences of Aspergillus fumigatus (Af) cpcA (GenBank XP_751584.1 ) and A. nidulans (An) cpcA (GenBank AF302935 ). Black boxes with white text denote amino acids identical in two of the three fungal species. Grey boxes with white text mark conserved changes. Gaps are introduced to optimize alignment. C) Alignment of the deduced amino acid sequence of the C-terminus conserved leucine zipper region with that of A. fumigatus CpcA and A. nidulans CpcA. The thick black line denotes the bZIP transcription factors basic domain signature (PS00036). Asterisks mark positions where conserved leucine residues characteristic of a leucine zipper (L-x(6)-L-x(6)-L-x(6)-L) should be found.

    Journal: BMC Microbiology

    Article Title: The cross-pathway control system regulates production of the secondary metabolite toxin, sirodesmin PL, in the ascomycete, Leptosphaeria maculans

    doi: 10.1186/1471-2180-11-169

    Figure Lengend Snippet: A) The cpcA locus of Leptosphaeria maculans . The conserved leucine zipper region at the C-terminus of cpcA is dark grey. The open boxes indicate the upstream Open Reading Frames uORF1 and uORF2 in the 5' leader region. The black dot preceding them represents the putative cross-pathway control element (CPRE) whose sequence is 5'TGACTCA3'. B) Alignment of the deduced amino acid sequence of uORF2 with counterparts in the leader sequences of Aspergillus fumigatus (Af) cpcA (GenBank XP_751584.1 ) and A. nidulans (An) cpcA (GenBank AF302935 ). Black boxes with white text denote amino acids identical in two of the three fungal species. Grey boxes with white text mark conserved changes. Gaps are introduced to optimize alignment. C) Alignment of the deduced amino acid sequence of the C-terminus conserved leucine zipper region with that of A. fumigatus CpcA and A. nidulans CpcA. The thick black line denotes the bZIP transcription factors basic domain signature (PS00036). Asterisks mark positions where conserved leucine residues characteristic of a leucine zipper (L-x(6)-L-x(6)-L-x(6)-L) should be found.

    Article Snippet: The sequences of the resulting plasmids were compared to the draft genome sequence of L. maculans isolate JN3 (Genoscope and Unité de Recherche Génomique Info, France) and 10 kb regions flanking these DNA fragments were analysed by FGENESH for presence of ORFs.

    Techniques: Control, Sequencing

    Quantitative Reverse Transcription PCR analysis of (A) cpcA, trpC and aroC , (B) sirZ and sirP in wild type (wt) and a cpcA -silenced (cpcA-sil) isolate of Leptosphaeria maculans . Six replicates of each isolate were grown in Tinline for eight days and then mycelia were washed and then transferred to fresh Tinline media for 5 h with 5 mM 3AT (+) or without 3AT (-). RNA was isolated from all treatments, cDNA prepared and q RT-PCR carried out. Transcript level is normalised to that of actin. Values are means ± SE of triplicate reactions of three independent biological samples. Asterisks mark values that have a significant increase (p < 0.05) in mean transcription levels compared to controls without 3AT.

    Journal: BMC Microbiology

    Article Title: The cross-pathway control system regulates production of the secondary metabolite toxin, sirodesmin PL, in the ascomycete, Leptosphaeria maculans

    doi: 10.1186/1471-2180-11-169

    Figure Lengend Snippet: Quantitative Reverse Transcription PCR analysis of (A) cpcA, trpC and aroC , (B) sirZ and sirP in wild type (wt) and a cpcA -silenced (cpcA-sil) isolate of Leptosphaeria maculans . Six replicates of each isolate were grown in Tinline for eight days and then mycelia were washed and then transferred to fresh Tinline media for 5 h with 5 mM 3AT (+) or without 3AT (-). RNA was isolated from all treatments, cDNA prepared and q RT-PCR carried out. Transcript level is normalised to that of actin. Values are means ± SE of triplicate reactions of three independent biological samples. Asterisks mark values that have a significant increase (p < 0.05) in mean transcription levels compared to controls without 3AT.

    Article Snippet: The sequences of the resulting plasmids were compared to the draft genome sequence of L. maculans isolate JN3 (Genoscope and Unité de Recherche Génomique Info, France) and 10 kb regions flanking these DNA fragments were analysed by FGENESH for presence of ORFs.

    Techniques: Reverse Transcription, Isolation, Reverse Transcription Polymerase Chain Reaction

    Sirodesmin PL levels in culture filtrates of in wild type (wt) and a cpcA -silenced (cpcA-sil) isolate of Leptosphaeria maculans . Cultures were grown for eight days in Tinline media (8d) and the culture filtrate isolated and sirodesmin PL levels were quantified by HPLC. Mycelia were washed then transferred to fresh Tinline medium with water (+H 2 O) or 5 mM 3AT (+3AT), or Tinline medium with no carbon or nitrogen sources (-C/N) for a further eight days. Culture filtrates from the three treatments (+H 2 O, +3AT, -C/N) were extracted and sirodesmin PL levels were quantified by HPLC. Values are means ± SE of three independent biological samples. Asterisks mark values that have a significant increase or decrease (p < 0.05) in sirodesmin PL production compared to water controls (+H 2 O).

    Journal: BMC Microbiology

    Article Title: The cross-pathway control system regulates production of the secondary metabolite toxin, sirodesmin PL, in the ascomycete, Leptosphaeria maculans

    doi: 10.1186/1471-2180-11-169

    Figure Lengend Snippet: Sirodesmin PL levels in culture filtrates of in wild type (wt) and a cpcA -silenced (cpcA-sil) isolate of Leptosphaeria maculans . Cultures were grown for eight days in Tinline media (8d) and the culture filtrate isolated and sirodesmin PL levels were quantified by HPLC. Mycelia were washed then transferred to fresh Tinline medium with water (+H 2 O) or 5 mM 3AT (+3AT), or Tinline medium with no carbon or nitrogen sources (-C/N) for a further eight days. Culture filtrates from the three treatments (+H 2 O, +3AT, -C/N) were extracted and sirodesmin PL levels were quantified by HPLC. Values are means ± SE of three independent biological samples. Asterisks mark values that have a significant increase or decrease (p < 0.05) in sirodesmin PL production compared to water controls (+H 2 O).

    Article Snippet: The sequences of the resulting plasmids were compared to the draft genome sequence of L. maculans isolate JN3 (Genoscope and Unité de Recherche Génomique Info, France) and 10 kb regions flanking these DNA fragments were analysed by FGENESH for presence of ORFs.

    Techniques: Isolation